DNA
Optimized

Part:BBa_K4043009:Design

Designed by: Jie Wang   Group: iGEM21_United_Shanghai_HS   (2021-09-25)


MHETase expressed genes in frame


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 238
    Illegal SapI site found at 988


Design Notes

none


Source

Literature review; optimized design

References